Effects of cytokines on VEGF expression and secretion by human first trimester trophoblast cell line.
| Autor: | Choi SJ; Department of Microbiology, Institute of Basic Medical Science, Yonsei University, Wonju College of Medicine, Kangwon-do, Korea., Park JY, Lee YK, Choi HI, Lee YS, Koh CM, Chung IB |
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| Jazyk: | angličtina |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2002 Aug; Vol. 48 (2), pp. 70-6. |
| DOI: | 10.1034/j.1600-0897.2002.01071.x |
| Abstrakt: | Problem: The mechanism through which vascular endothelial growth factor (VEGF) regulation occurs at the feto-maternal interface is poorly understood. The aim of this study was to investigate the effects of various cytokines on VEGF expression and secretion by trophoblast cells. Method of Study: We investigated the effects of cytokines on VEGF expression in human first trimester trophoblast cell line by analyzing VEGF messenger RNA (mRNA) by reverse transcription-polymerase chain reaction and VEGF protein secretion by enzyme linked immunosorbent assay. Results: The trophoblast cells expressed VEGF mRNA constitutively and the main subtypes were identified as VEGF121 and VEGF165. When cultured in the presence of interferon (IFN)-gamma, interleukin (IL)- 1beta, tumor necrosis factor (TNF)-alpha, IL-2, or IL-10, VEGF mRNA expression was found to be significantly increased by IL-1beta, IFN-gamma and TNF-alpha but to be unaffected by IL-2 and IL-10. Moreover, VEGF secretion was most significantly increased by IFN-gamma treatment. Conclusion: These results suggest that IL-1beta, IFN-gamma, and TNF-alpha may regulate the production of VEGF in early gestational trophoblasts. |
| Databáze: | MEDLINE |
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