| Autor: |
Ikeda M; Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja 719-1197, Japan. mikeda@fhw.oka-pu.ac.jp, Umami K, Hinohara M, Tanimura Y, Ohmae A, Nakanishi Y, Maeshima M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of experimental botany [J Exp Bot] 2002 Nov; Vol. 53 (378), pp. 2273-5. |
| DOI: |
10.1093/jxb/erf074 |
| Abstrakt: |
The function of the translation product of cDNA for Acetabularia vacuolar H(+)-pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3-deficient strain. The open reading frame of Acetabularia H(+)-pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous paper). The acidification of the vacuole was observed by fluorescence microscopy when the cDNA was constructed in pYES2. Immunoblot analysis also supported the localization of the translation product in the vacuolar-membrane-enriched fraction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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