| Autor: |
Bryan CF; Midwest Transplant Network, Westwood, KS 66205, USA., McDonald SB, Baier KA, Luger AM, Aeder MI, Murillo D, Muruve NA, Nelson PW, Shield CF 3rd, Warady BA |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical transplantation [Clin Transplant] 2002; Vol. 16 Suppl 7, pp. 15-23. |
| DOI: |
10.1034/j.1399-0012.16.s7.2.x |
| Abstrakt: |
HLA Class I antibody screening can be performed by flow cytometry using a mixture of 30 distinct bead populations each coated with the Class I antigen phenotype derived from different cell lines. In this study we compared the efficacy of Class I antibody screens done by flow cytometry beads with the antihuman globulin (AHG) method for patients awaiting cadaveric renal retransplantation. Class I panel reactive antibody (PRA) screening by flow cytometric beads of 21 regraft serum samples that had all been found to be negative by AHG DTT Class I PRA, revealed that 57.1% (12 of 21) had a flow Class I PRA of > or = 10%. Furthermore, when five regraft sera with an intermediate PRA were screened (mean AHG DTT PRA = 33.2 +/- 13%) the mean flow Class I PRA almost doubled (mean flow PRA = 72.4 +/- 10.2%) (p < 0.01). When active UNOS waiting list regraft candidates, after several months of screening the Class I PRA by flow beads, were divided into the three PRA categories based on their peak flow Class I PRA value (0-20%, 21-79% and > or = 80%), the incidence of a positive flow cross-match was 0%, 72% and 85% and the incidence of retransplantation was 60%, 22% and 10%, in each of these groups, respectively. These data provided our histocompatibility laboratory with the rationale to stop performing the AHG PRA and perform only the flow Class I PRA method for regraft candidates. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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