pSVPoMcat modifying Schwann cell to protect injured spinal neurons in rats.
| Autor: | Chen L; Department of Neurosurgery, General Hopital of Chengdu Military Command, Chengdu 610083, China., Gao L, Piao Y, Mao B, Zeng F |
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| Jazyk: | angličtina |
| Zdroj: | Chinese journal of traumatology = Zhonghua chuang shang za zhi [Chin J Traumatol] 2002 Oct; Vol. 5 (5), pp. 316-20. |
| Abstrakt: | Objective: To investigate the protective effect of pSVPoMcat (myelin basic protein microgene) modifying Schwann cell on injured spinal neurons. Methods: A model of rat spinal cord injured by hemisection was used. One hundred and twenty healthy SD rats of both sexes weighing 250-300 g were divided into three groups: Group A (n=40, treated with implantation of pSVPoMcat modifying Schwann cell), Group B (n= 40, treated with implantation of Schwann cell only) and Group C (n=400, treated with sham operation as the control). One week after operation the rat functional recovery was observed dynamically by using combined behavioral score (CBS) and cortical somatasensory evoked potentials, the spinal cord sections were stained by Nissl, acid phosphatase enzyme histochemistry and cell apoptosis was examined by methye green, terminal deoxynucleotidyl and the dUTP Nick end labeling technique. Quantitative analysis was done by computer image analysis system. Results: In Group A the injured neurons recovered well morphologically. The imaging analysis showed a result of Group A |
| Databáze: | MEDLINE |
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