| Autor: |
Melchiorre MN; Instituto de Fitopatología y Fisiología Vegetal, (IFFIVE-INTA), Camino 60 Cuadras Km 5o, (X5020ICA) Córdoba, Argentina. mmelchiorre@correo.inta.gov.ar, Lascano HR, Trippi VS |
| Jazyk: |
angličtina |
| Zdroj: |
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al [Biocell] 2002 Aug; Vol. 26 (2), pp. 217-23. |
| Abstrakt: |
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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