Autor: |
van Rooij EM; Central Institute for Animal Disease Control, CIDC-Lelystad, PO Box 2004, Houtribweg 39, NL 8203 AA Lelystad, The Netherlands. e.m.a.vanrooij@id.wag-ur.nl, Glansbeek HL, Hilgers LA, te Lintelo EG, de Visser YE, Boersma WJ, Haagmans BL, Bianchi AT |
Jazyk: |
angličtina |
Zdroj: |
Journal of virology [J Virol] 2002 Oct; Vol. 76 (20), pp. 10540-5. |
DOI: |
10.1128/jvi.76.20.10540-10545.2002 |
Abstrakt: |
To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants dimethyldioctadecylammonium bromide in water (DDA) and sulfolipo-cyclodextrin in squalene in water. We demonstrate that a DNA vaccine combined with DDA, but not with the other adjuvants, induced significantly stronger immune responses than plasmid vaccination alone. Moreover, pigs vaccinated in the presence of DDA were protected against clinical disease and shed significantly less PRV after challenge infection. This is the first study to demonstrate that DDA, a conventional adjuvant, enhances DNA vaccine-induced antiviral immunity. |
Databáze: |
MEDLINE |
Externí odkaz: |
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