| Autor: |
Lis AW, Mclaughlin RK, Mclaughlin DI |
| Jazyk: |
angličtina |
| Zdroj: |
Physiological chemistry and physics [Physiol Chem Phys] 1975; Vol. 7 (6), pp. 565-70. |
| Abstrakt: |
Yeast ribosomal RNA was hydrolyzed to its constituent nucleosides with the aid of snake venom and bacterial alkaline phosphatase. Lyophilized hydrolysate was labeled with radioactive 5-hydroxyuridine and applied to partition chromatography. It was found that some components of rRNA are held on the column and can be eluted with water. Eighty-nine percent of the label, a large portion of cytidine, and several unidentified compounds were found in the water wash. The direct application of the wash concentrate to ascending paper chromatography in saturated butanol-H2O resulted in the separation of three distinct UV-absorbing bands. Further resolution and characterization of one band of unidentified material revealed the presence of an additional nucleoside. On the basis of chromatographic and electrophoretic behavior and UV-absorption spectra, it was tentatively identified as 5-hydroxymethyluridine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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