MCP-1 is highly expressed in peritoneum following midline laparotomy with peritoneal abrasion in a murine model.

Autor: Brodsky JA; Minimally Invasive Surgery Center and Department of Immunology, the Cleveland Clinic Foundation, Cleveland, OH 44195, USA. brodyf@ccf.org, Brody FJ, Endlich B, Armstrong DA, Ponsky JL, Hamilton IA
Jazyk: angličtina
Zdroj: Surgical endoscopy [Surg Endosc] 2002 Jul; Vol. 16 (7), pp. 1079-82. Date of Electronic Publication: 2002 Apr 09.
DOI: 10.1007/s00464-001-8335-z
Abstrakt: Background: Monocyte chemoattractant protein-1 (MCP-1), a CC chemokine, is a potent attractant of monocytes both in vitro and in vivo. However, its role in the repair of peritoneal injury is not well established. This study characterizes MCP-1 expression in surgical wounds following peritoneal abrasion in a murine model.
Methods: Twenty-five C57 BL6 female mice underwent a 2-cm midline laparotomy with mechanical abrasion of the right peritoneal wall. The mice were sacrificed at various times ranging from 0 to 7 days. Hemotoxylin and eosin stained sections and tissue extracts were made using peritoneal samples from abraded and unabraded areas in each mouse. An enzyme-linked immunosorbent assay was performed on the specimens to quantitate MCP-1 expression. Values were compared using a t-test.
Results: At baseline, there was minimal expression of MCP-1 (<5 pg/mg protein). Following surgery, MCP-1 levels at abraded sites were significantly higher than those at both baseline and unabraded sites at all times up to a week following surgery. Histologic evaluation revealed peritoneal thickening and leukocytic infiltration of only abraded surfaces.
Conclusion: MCP-1 is highly expressed in peritoneum following laparotomy with peritoneal abrasion. Elevations in MCP-1 levels are identified within 6 h of surgery and persist for up to 1 week. The histologic differences between abraded and unabraded areas may be attributable to differences in MCP-1 expression. Further studies using recombinant MCP-1 and anti-MCP-1 antibody may elucidate this relationship.
Databáze: MEDLINE
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