[Isolation of chloroplasts and chloroplast DNA from pea leaves].

Autor: Amerkhanova MB, Boguspaev KK, Sliusarenko AG
Jazyk: ruština
Zdroj: Biokhimiia (Moscow, Russia) [Biokhimiia] 1975 Nov-Dec; Vol. 40 (6), pp. 1312-4.
Abstrakt: A method of isolating DNA from pea chloroplasts (ch-DNA) in CsCl density gradient is described. DNA preparations are free of 5-methylcytosine and have a melting temperature of 86.5 degrees. Denatured DNA molecules completely reassociate for 3 hours at 60 degrees C. It is concluded that the preparations obtained are pure ch-DNA.
Databáze: MEDLINE