| Autor: |
Vercruysse KP; Department of Medicinal Chemistry, The University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108, USA., Li H, Luo Y, Prestwich GD |
| Jazyk: |
angličtina |
| Zdroj: |
Biomacromolecules [Biomacromolecules] 2002 Jul-Aug; Vol. 3 (4), pp. 639-43. |
| DOI: |
10.1021/bm020026d |
| Abstrakt: |
Addition of trivalent lanthanide salts to solutions of hyaluronan (HA), a naturally occurring anionic polysaccharide, resulted in an unexpected reverse-temperature phase transition (RTPT); i.e., the complexes were soluble when cooled but precipitated when warmed. A unique lower critical solution transition temperature (LCST) was observed for each lanthanide in the order (increasing LCST) Eu(3+) < Nd(3+)< Ce(3+) < Gd(3+) < La(3+) < Tb(3+) < Dy(3+) < Yb(3+) < Lu(3+) for high molecular weight HA. Moreover, the LCST values increased as the molecular weight of the HA decreased from 1730 to 190 kDa. The precipitation-dissolution behavior was fully reversible in a cyclical fashion; a lag in redissolution was observed as the temperature was lowered, and longer incubation times at each temperature minimized this lag. The RTPT behavior could be ablated by titration with 8 kDa HA to produce complexes soluble at ambient temperature. This is the first description of RTPT behavior in a biologically important glycosaminoglycan. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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