| Autor: |
Perkovskaia GIu; Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kyiv. gperk@uninet.kiev.ua, Kravchuk ZhN, Dmitriev AP |
| Jazyk: |
ruština |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2001 Sep-Oct; Vol. 73 (5), pp. 108-13. |
| Abstrakt: |
The elicitor-induced generation of two oxygen species in tomato cell culture as well as their involvement into hypersensitive reaction was investigated. Generation of superoxide O2.- was measured by a lucigenin-related chemiluminescence. Accumulation of hydrogen peroxide H2O2 was measured by a fluorescent probe pyranin. Xylanase and chitosan were used as biotic elicitors with different mode of action. It was found that both O2.- and H2O2 had been accumulated in elicitor-treated tomato cells. The results obtained show that reactive oxidants are important signal transduction elements for activation of hypersensitive response in tomato cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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