In vitro studies on a bioreactor module containing encapsulated goat hepatocytes for the development of bioartificial liver.
| Autor: | Khan AA; Center for Liver Diseases, Owaisi Hospital and Research Center, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad. aleem_a_khan@rediffmail.com, Capoor AK, Parveen N, Naseem S, Venkatesan V 5th, Habibullah CM |
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| Jazyk: | angličtina |
| Zdroj: | Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology [Indian J Gastroenterol] 2002 Mar-Apr; Vol. 21 (2), pp. 55-8. |
| Abstrakt: | Background: A bioartificial liver may act as a temporary metabolic bridge in patients with acute liver failure. We devised a bioreactor module containing encapsulated goat hepatocytes and studied its efficiency in detoxifying ammonia. Methods: A hollow-glass bioreactor module was designed and fabricated locally. The module was inoculated with alginate poly-L-lysine microcapsules with entrapped goat hepatocytes. Metabolism of ammonia and glucose was assessed. Antibody-mediated cell cytotoxicity was also assessed. Results: The optimum encapsulated goat hepatocyte concentration was 12-18 billion at a perfusate flow rate of 30 mL/min under oxygenated condition. The optimum ammonium chloride concentration for detoxification was 2.5-5.0 mM. There was little or no cytolysis of encapsulated hepatocytes on exposure to complement-inactivated human AB serum. Conclusion: Encapsulated goat hepatocytes efficiently detoxified ammonia to urea. Cells were metabolically active up to 48 hours, indicating their feasibility for use in a bioreactor module. Encapsulation protected the hepatocytes from antibody-mediated cell lysis. |
| Databáze: | MEDLINE |
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