Autor: |
Markarian ShA; Department of Chemistry, Yerevan State University, Yerevan, 375049 Armenia., Bagramian KA, Arakelian VB |
Jazyk: |
ruština |
Zdroj: |
Biofizika [Biofizika] 2002 Mar-Apr; Vol. 47 (2), pp. 315-7. |
Abstrakt: |
It was shown by the method of penetrating tetraphenylphosphonium cations that low-temperature freezing (-196 degrees C) of Escherichia coli leads to a sharp decrease (from 198 to 85 mV) in membrane potential. Incubation of bacteria in a medium containing dimethyl sulfoxide and diethyl sulfoxide as cryoprotectors results in a reduction of the potential by 16 and 27 mV, respectively. It was also shown that diethyl sulfoxide is more effective in maintaining the membrane potential after freezing--thawing than dimethyl sulfoxide. |
Databáze: |
MEDLINE |
Externí odkaz: |
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