| Autor: |
Guo J; Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA., Hawwari A, Li H, Sun Z, Mahanta SK, Littman DR, Krangel MS, He YW |
| Jazyk: |
angličtina |
| Zdroj: |
Nature immunology [Nat Immunol] 2002 May; Vol. 3 (5), pp. 469-76. Date of Electronic Publication: 2002 Apr 22. |
| DOI: |
10.1038/ni791 |
| Abstrakt: |
T cell receptor (TCR) alpha alleles undergo primary and secondary rearrangement in double-positive (DP) thymocytes. By analyzing TCRalpha rearrangement in orphan nuclear receptor RORgamma-deficient mice, in which the DP lifespan is shorter, and in Bcl-x(L)-transgenic mice, in which the DP lifespan is extended, we show that the progression of secondary V(alpha) to J(alpha) rearrangements is controlled by DP thymocyte survival. In addition, because Bcl-x(L) induces a bias towards 3' J(alpha) usage in peripheral T cells, we conclude that the programmed cell death of DP thymocytes is not simply a consequence of failed positive selection. Rather, it limits the progression of rearrangement along the J(alpha) locus and the opportunities for positive selection, thereby regulating the TCRalpha repertoire. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
