| Autor: |
Yu YY; Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA., Netuschil N, Lybarger L, Connolly JM, Hansen TH |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Apr 01; Vol. 168 (7), pp. 3145-9. |
| DOI: |
10.4049/jimmunol.168.7.3145 |
| Abstrakt: |
We report in this work the expression and characterization of class I molecules expressed as single-chain trimers consisting of an antigenic peptide-spacer-beta(2)-microglobulin-spacer H chain. Our results indicate that these single-chain constructs assemble efficiently, maintain their covalent structure, and are unusually stable at the cell surface. Consequently, these constructs are at least 1000-fold less accessible to exogenous peptide than class I molecules loaded with endogenous peptides, and they are potent simulators of peptide-specific CTL and Abs. Our combined findings suggest that single-chain trimers may have applications as DNA vaccines against virus infection or tumors. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
