| Autor: |
Palman KP; Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand. k.p.palmano@massey.ac.nz, Elgar DF |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. A [J Chromatogr A] 2002 Feb 22; Vol. 947 (2), pp. 307-11. |
| DOI: |
10.1016/s0021-9673(01)01563-1 |
| Abstrakt: |
An existing RP-HPLC method for the measurement of the major bovine whey proteins in bovine whey samples and powders has been extended to include the analysis of the minor bovine whey protein lactoferrin. Lactoferrin could be detected and quantitated at levels down to 0.2 microg and linear calibration was observed between 0.2 and 30 microg. Reliable quantitation of lactoferrin in whey samples could be achieved provided the bovine serum albumin to lactoferrin ratio did not exceed 10:1. Quantitative data obtained by the RP-HPLC method compared favourably with data obtained by Mono S analytical chromatography. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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