A cytochemical method for visualising blood vessels selectively in semithin LR white sections.

Autor: von Ruhland CJ; Medical Microscopy Sciences, University of Wales College of Medicine, Cardiff, UK., Newman GR
Jazyk: angličtina
Zdroj: The Histochemical journal [Histochem J] 2001 Jul; Vol. 33 (7), pp. 433-5.
DOI: 10.1023/a:1013732112957
Abstrakt: Traditional cytochemical techniques for illustrating blood vessels are usually unsuitable for use in high resolution, 0.35 microm-thick LR White sections due to the thinness of the tissue and, if collagenous, high background staining. We report that a modification of the periodic acid-thiocarbohydrazide-silver proteinate-silver enhancement method (PATCH-SP-SE) selectively visualises, with low background, even the smallest blood vessels in semithin LR White sections of highly collagenous tissues such as human parietal peritoneum.
Databáze: MEDLINE