| Autor: |
Elisseeff J; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA., McIntosh W, Fu K, Blunk BT, Langer R |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2001 Nov; Vol. 19 (6), pp. 1098-104. |
| DOI: |
10.1016/S0736-0266(01)00054-7 |
| Abstrakt: |
Photopolymerizing hydrogel systems provide a method to encapsulate cells and implant materials in a minimally invasive manner. Controlled release of growth factors in the hydrogels may enhance the ability to engineer tissues. IGF-I and TGF-beta1 were loaded in PLGA microspheres using a double emulsion technique. 125 ng and 200 pg of active IGF-I and TGF-beta, respectively, as measured by ELISA, were released over 15 days. The growth factor containing microspheres were photoencapsulated with bovine articular chondrocytes in PEO-based hydrogels and incubated in vitro for two weeks. Statistically significant changes in glycosaminoglycan (GAG) production compared to control gels either without microspheres or with blank spheres were observed after a 14 day incubation with IGF-I and IGF-I/TGF-beta microspheres combined, with a maximum density of 8.41+/-2.5% wet weight GAG. Total collagen density was low and decreased with the IGF-I/TGF-beta microspheres after two weeks incubation, but otherwise remained unchanged in all other experimental groups. Cell content increased 10-fold to 0.18+/-0.056 x 10(6) cells/mg wet weight and extracellular matrix (ECM) staining by H&E increased in hydrogels with IGF-I/TGF-beta microspheres. In conclusion, photoencapsulation of microspheres in PEO-based hydrogels provides a method to deliver molecules such as growth factors in porous hydrogel systems. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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