Positive ion electrospray ionization mass spectrometry of double-stranded DNA/drug complexes.

Autor: Gupta R; Department of Chemistry, University of Wollongong, NSW 2522, Australia., Kapur A, Beck JL, Sheil MM
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2001; Vol. 15 (24), pp. 2472-80.
DOI: 10.1002/rcm.524
Abstrakt: Positive ion electrospray ionization mass spectra of 16 base-pair double-stranded (ds)DNA have been obtained with essentially no ions from single-stranded DNA present. Single-stranded DNA was minimized by: (1) careful choice of DNA sequences; (2) the use of a relatively high salt concentration (0.1 M ammonium acetate, pH 8.5), and, (3) a low desolvation temperature (40 degrees C). Similarly, ESI-MS complexes of dsDNA with cisplatin, daunomycin and distamycin were obtained that contained only negligible amounts of single-stranded DNA. The complexes with daunomycin and distamycin were more stable to strand separation in the gas phase than dsDNA alone. This is in agreement with solution studies and with other recent gas phase results. These data contrast with many earlier ESI-MS studies of dsDNA and DNA/drug complexes in which ions from ssDNA are also normally observed.
(Copyright 2001 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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