Molecular weight, structure, and shape of oat (1-->3),(1-->4)-beta-D-glucan fractions obtained by enzymatic degradation with lichenase.
| Autoři: | Roubroeks JP; Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, SE-750 07 Uppsala, Sweden., Mastromauro DI, Andersson R, Christensen BE, Aman P |
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| Zdroj: | Biomacromolecules [Biomacromolecules] 2000 Winter; Vol. 1 (4), pp. 584-91. |
| Způsob vydávání: | Journal Article; Research Support, Non-U.S. Gov't |
| Jazyk: | English |
| Informace o časopise: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print Cited Medium: Print ISSN: 1525-7797 (Print) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Chemical Society, c2000- |
| Výrazy ze slovníku MeSH: | beta-Glucans*, Avena/*chemistry , Glucans/*chemistry , Glycoside Hydrolases/*chemistry, Chromatography, Gel ; Chromatography, High Pressure Liquid ; Hydrolysis ; Lasers ; Magnetic Resonance Spectroscopy ; Scattering, Radiation ; Viscosity |
| Abstrakt: | Oat beta-glucan was partially degraded by lichenase for different periods of time. Weight-average molecular weights were obtained from SEC-RI-RALLS-Visc and ranged from 5300 to 257,200. Weight-average intrinsic viscosities ranged from 7 to 160 mL/g. The determined viscosity equation, [eta] = 5.47 x 10(-3) M 0.82, indicated an extended coil conformation. When the coil was modeled as a wormlike chain a persistent length of 2.30 nm was obtained. 1H and 13C NMR spectroscopy were used to identify the structural units in the partially degraded beta-glucan. The identification of the disaccharide laminaribiose in the hydrolysates may suggest the presence of a new structural feature in cereal beta-glucan. |
| Substance Nomenclature: | 0 (Glucans) 0 (beta-Glucans) 55965-23-6 (beta-glucan, (1-3)(1-4)-) EC 3.2.1.- (Glycoside Hydrolases) EC 3.2.1.73 (licheninase) |
| Entry Date(s): | Date Created: 20011117 Date Completed: 20020117 Latest Revision: 20191105 |
| Update Code: | 20240829 |
| DOI: | 10.1021/bm000017+ |
| PMID: | 11710185 |
| Autor: | Roubroeks JP; Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, SE-750 07 Uppsala, Sweden., Mastromauro DI, Andersson R, Christensen BE, Aman P |
| Jazyk: | angličtina |
| Zdroj: | Biomacromolecules [Biomacromolecules] 2000 Winter; Vol. 1 (4), pp. 584-91. |
| DOI: | 10.1021/bm000017+ |
| Abstrakt: | Oat beta-glucan was partially degraded by lichenase for different periods of time. Weight-average molecular weights were obtained from SEC-RI-RALLS-Visc and ranged from 5300 to 257,200. Weight-average intrinsic viscosities ranged from 7 to 160 mL/g. The determined viscosity equation, [eta] = 5.47 x 10(-3) M 0.82, indicated an extended coil conformation. When the coil was modeled as a wormlike chain a persistent length of 2.30 nm was obtained. 1H and 13C NMR spectroscopy were used to identify the structural units in the partially degraded beta-glucan. The identification of the disaccharide laminaribiose in the hydrolysates may suggest the presence of a new structural feature in cereal beta-glucan. |
| Databáze: | MEDLINE |
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