| Autor: |
Julio SM; Department of Molecular, University of California, Santa Barbara, California 93106, USA., Heithoff DM, Provenzano D, Klose KE, Sinsheimer RL, Low DA, Mahan MJ |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 2001 Dec; Vol. 69 (12), pp. 7610-5. |
| DOI: |
10.1128/IAI.69.12.7610-7615.2001 |
| Abstrakt: |
Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. Overproduction of Dam significantly attenuated the virulence of these two pathogens, leading to, in Yersinia, the ectopic secretion of virulence proteins (Yersinia outer proteins) and a fully protective immune response in vaccinated hosts. Dysregulation of Dam activity may provide a means for the development of vaccines against varied bacterial pathogens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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