| Autor: |
Patni AK; Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India., Gupta S, Sharma A, Tiwary AK, Garg SK |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2001 Oct; Vol. 53 (10), pp. 1387-92. |
| DOI: |
10.1211/0022357011777738 |
| Abstrakt: |
The Na+-Ca2+ exchanger and Ca2+-ATPase pumps reported to be present on the sperm membrane are responsible for maintaining the intracellular Ca2+ concentration that is involved in regulation of sperm function. We have investigated the role of intracellular Ca2+ in the presence of 2',4'-dichlorobenzamil hydrochloride (benzamil), a Na+-Ca2+ exchange inhibitor, on human sperm motility. The mechanism of the complementary spermicidal action produced by a combination of benzamil and propranolol on human spermatozoa has been investigated also. When administered alone benzamil and propranolol produced a dose- and time-dependent decrease in motility of sperm in ejaculated semen and spermatozoa separated from semen. A combination of benzamil and propranolol exhibited a complementary spermicidal action, thereby resulting in dose reduction of both drugs for obtaining total immotility within 1 min of administration. An increase in the intracellular Ca2+ level was found to contribute to the spermicidal activity. Inhibition of the Na+-Ca2+ exchange system on sperm membrane by benzamil and membrane stabilization by propranolol resulted in accumulation of Ca2+ inside the sperm cells. When the two drugs were used in combination the time required for the total loss of motility of spermatozoa was significantly reduced due to a similar mechanism of action of both drugs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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