Effect of 7beta-hydroxycholesterol on cellular redox status and heat shock protein 70 expression in macrophages.
| Autor: | Bansal G; Department of Biophysics, Panjab University, Chandigarh-160014, India. mpbansal@pu.ac.in, Singh U, Bansal MP |
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| Jazyk: | angličtina |
| Zdroj: | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2001; Vol. 11 (5), pp. 241-6. |
| DOI: | 10.1159/000047809 |
| Abstrakt: | The proatherogenic effects of oxidized low-density lipoproteins are attributed to oxysterols, particularly 7beta-hydroxycholesterol. In the present study, we have examined the effect of 7beta-hydroxycholesterol on cellular redox status and heat shock protein 70 (hsp70) expression. Murine peritoneal macrophages were incubated for 24 hours with increasing concentrations of 7beta-hydroxycholesterol (0-25 microg/ml). Intracellular reduced glutathione levels decreased and oxidized glutathione levels increased, thus increasing the redox ratio. Hsp70 expression in macrophages decreased in a dose-dependent fashion. It is suggested that 7beta-hydroxycholesterol exerts oxidative stress on macrophages and makes them more susceptible to its proatherogenic effects by impairing the cellular defense system consisting of antioxidants and stress proteins. (Copyright 2001 S. Karger AG, Basel) |
| Databáze: | MEDLINE |
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