| Autor: |
Davis MT; Department of Biochemistry and Genetics, Amgen, Thousand Oaks, CA, USA., Spahr CS, McGinley MD, Robinson JH, Bures EJ, Beierle J, Mort J, Yu W, Luethy R, Patterson SD |
| Jazyk: |
angličtina |
| Zdroj: |
Proteomics [Proteomics] 2001 Jan; Vol. 1 (1), pp. 108-17. |
| DOI: |
10.1002/1615-9861(200101)1:1<108::AID-PROT108>3.0.CO;2-5 |
| Abstrakt: |
With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spectrometer. Precursor acquisition rates of up to 20 distinct precursors/minute in a single analysis were obtained through the use of parallel precursor selection (four precursors/survey period) and variable collision induced dissociation integration time (1 to 6 periods summed). Maximal exploitation of the gas phase fractionated ions was obtained through the use of narrow survey scans and iterative data-dependent analyses incorporating dynamic exclusion. The impact on data fidelity as a product of data-dependent selection of precursor ions from a dynamically excluded field is discussed with regards to sample complexity, precursor selection rates, survey scan range and facile chemical modifications. Operational and post-analysis strategies are presented to restore data confidence and reconcile the greatest number of matched spectra. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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