| Autor: |
Jeon EJ; Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul., Park YS, Choi YC, Yeo SW, Jung TT |
| Jazyk: |
angličtina |
| Zdroj: |
The Annals of otology, rhinology, and laryngology [Ann Otol Rhinol Laryngol] 2001 Oct; Vol. 110 (10), pp. 917-21. |
| DOI: |
10.1177/000348940111001005 |
| Abstrakt: |
Tumor necrosis factor (TNF)-alpha is important in the pathogenesis of otitis media with effusion (OME). The purpose of this study was to determine the effect of TNF-alpha antagonist on the outcome of lipopolysaccharide (LPS)-induced OME in rats. Otitis media was induced by injecting Pseudomonas aeruginosa LPS transtympanically. Another (combination) group was pretreated with TNF-alpha antagonist, soluble TNF receptor type I (sTNF RI), before transtympanic injection of LPS. Saline and phosphate-buffered saline solutions were used as controls. Twelve hours after the transtympanic injection, otoscopic examination and aspiration of middle ear effusion (MEE) were done. The temporal bones in each group were examined histopathologically, and the vascular permeability of the middle ear mucosa was measured by the Evans blue vital dye technique. In the LPS and combination groups, MEE developed in 90% and 0% of ears, respectively. The combination group showed less inflammation, less mucosal thickening, and significantly decreased vascular permeability as compared to the LPS group. Transtympanic administration of sTNF RI appears to suppress the development of LPS-induced OME. This study suggests that TNF-alpha antagonist, along with antibiotics, may have an adjunctive role in the future treatment of MEE. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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