| Autor: |
Wintzen M; Department of Dermatology, Leiden University Medical Center, The Netherlands. mwintzen@knmg.nl, de Winter S, Out-Luiting JJ, van Duinen SG, Vermeer BJ |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental dermatology [Exp Dermatol] 2001 Oct; Vol. 10 (5), pp. 305-11. |
| DOI: |
10.1034/j.1600-0625.2001.100502.x |
| Abstrakt: |
The production and its induction by ultraviolet radiation (UVR) of proopiomelanocortin (POMC)-derived peptides by keratinocytes has been reported, albeit not consistently. Recently we demonstrated that only under specific culturing conditions human keratinocytes are capable of producing a beta-endorphin (betaE)-like peptide with the characteristics of beta-lipotropin (betaLPH). Here the presence and UV-induction of betaE-immunoreactivity (betaE-IR) in keratinocytes in human skin in vivo was investigated. betaE-IR was detectable by immunohistochemistry in keratinocytes of the follicular matrix and to some extent in cells of sweat ducts, but was absent from epidermal keratinocytes. Absence of betaE-IR was confirmed by radioimmunoassay of HPLC-fractionated extracts of normal epidermis. Repeated exposure to solar-simulated UVR had no effect. This investigation is the first to demonstrate the presence of betaE-immunoreactive material in the follicular matrix of corporal hairs and in duct cells of sweat glands. The possible meaning of these results is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
