| Autor: |
Clifton MJ; Laboratoire de Genie Chimique, Universite Paul Sabatier, Toulouse, France., Roux-de Balmann H, Sanchez V |
| Jazyk: |
angličtina |
| Zdroj: |
AIChE journal. American Institute of Chemical Engineers [AIChE J] 1996 Jul; Vol. 42 (7), pp. 2069-79. |
| DOI: |
10.1002/aic.690420727 |
| Abstrakt: |
During the IML-2 space shuttle mission, the RAMSES instrument was operated in the Spacelab module. This continuous-flow electrophoresis device performs separation and purification of protein solutions on a preparative scale. Samples containing artificial mixtures of pure proteins were used to test the capabilities of the device, and useful separations were obtained for proteins having a mobility difference of only 3 x 10(-9) m2 V-1 s-1. Operating conditions that cannot be applied on earth were explored for two different sample concentrations, one of which is too high to allow treatment on earth. It agrees well with a previously published numerical model in that the main cause of loss in resolution in this process is the electrohydrodynamic spreading of the protein filaments. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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