| Autor: |
Francoeur SN; Department of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama 35487-0206, USA. steven_francoeur@hotmail.com, Wetzel RG, Neely RK |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2001 Sep; Vol. 67 (9), pp. 4329-34. |
| DOI: |
10.1128/AEM.67.9.4329-4334.2001 |
| Abstrakt: |
A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dissolved-substrate assay detected similar responses of biofilm extracellular protease activity to experimental manipulation of N supply. Combination of this technique with confocal scanning laser microscopy allowed direct visualization of microspatial patterns of bacterial distribution and extracellular protease activity at the biofilm-substratum interface. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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