Microvascular transplantation and replantation of the rabbit submandibular gland.

Autor:	Spiegel JH; Department of Otolaryngology-Head and Neck Surgery, Boston University School of Medicine, D-616, 88 E Newton St, Boston, MA 02118, USA. jeffrey.spiegel@bmc.org, Deschler DG, Cheney ML
Jazyk:	angličtina
Zdroj:	Archives of otolaryngology--head & neck surgery [Arch Otolaryngol Head Neck Surg] 2001 Aug; Vol. 127 (8), pp. 991-6.
DOI:	10.1001/archotol.127.8.991
Abstrakt:	Background: Xerostomia is a devastating complication of radiation therapy. Previous research has demonstrated that submandibular glands may be removed from the neck and transplanted using microvascular techniques, with good gland survival. However, microvascular transplantation and replantation has never been attempted on a composite tissue such as a salivary gland. Objective: To evaluate the ability of a rabbit submandibular gland to undergo 2 successive microvascular transplantations. Subjects and Design: Study rabbits underwent a midline neck incision with dissection of a submandibular gland to its arterial and venous pedicle. Microvascular techniques were then used to transplant the gland to the femoral system of the right groin. The incisions were reopened later under surgical conditions. The transferred gland was examined for survival and patency of its artery and vein. Healthy glands were dissected and transferred to a suitable artery and vein within the neck, where they were again reanastamosed using microvascular surgical techniques. After additional time, the gland was again examined for survival and pedicle patency, then removed and evaluated for histopathological evidence of survival. Results: Surgical technique evolved during the course of this work to avoid encountered pitfalls. After refining the technique, we have determined that the rabbit submandibular gland is able to withstand successive microvascular transplantation and replantation with good likelihood of long-term survival, according to histopathological criteria. Conclusions: The rabbit submandibular gland is able to undergo microvascular transplantation and replantation with evidence of long-term survivability and preserved function. The body's natural response to surgery and tissue transplantation makes replantation a technical challenge; however, methods delineated herein alleviate many of the potential pitfalls. Extending these results to humans, patients who are to undergo radiation therapy could have a disease-free gland removed from the neck, transferred outside of the field of radiation, and then returned to the neck at the completion of radiation therapy. This may enable them to maintain salivary gland function and maintain oral cavity function and comfort.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu