1,25(OH)2 vitamin D concentration in the plasma of Solanum glaucophyllum intoxicated rabbits.

Autor: Dallorso ME; School of Agricultural Sciences, Lomas de Zamora National University, Provincia de Buenos Aires, Republica Argentina. carou@ciudad.com.ar, Gil S, Pawlak E, Lema F, Márquez A
Jazyk: angličtina
Zdroj: Australian veterinary journal [Aust Vet J] 2001 Jun; Vol. 79 (6), pp. 419-23.
DOI: 10.1111/j.1751-0813.2001.tb12987.x
Abstrakt: Objective: To test Solanum glaucophyllum calcinotic effects in adult New Zealand White rabbits in relation to cumulative dose and active principle concentration in plasma.
Design: An intoxication assay with controls.
Procedure: Rabbits were orally dosed with aqueous extracts of dry leaves of S glaucophyllum for 5, 7 or 9 days. During the experiment, body weight, calcaemia and phosphataemia were measured; retinal blood vessel calibre was observed by ophthalmoscopic examination of the ocular fundus. 1,25(OH)2 vitamin D plasma concentration was determined at the end of the experimental periods. Soft tissue calcium concentration and the presence of calcinotic lesions were studied after euthanasia.
Results: Toxic effects were evident in S glaucophyllum treated groups (loss of body weight, elevation of soft tissue calcium concentration, and presence of calcinotic lesions). Plasma 1,25(OH)2 vitamin D concentrations were negatively correlated with final body weight (r = -0.97; P < or = 0.001), and positively correlated with renal calcium concentration (r = 0.74; P = 0.02). There was also a significant regression of plasma 1,25 (OH)2 vitamin D concentration on the cumulative dose of S glaucophyllum (R2 = 0.87; P < or = 0.001).
Conclusions: The procedure described here offers a sensitive and practical experimental model for the study of the pathogenesis of enteque seco.
Databáze: MEDLINE
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