Co-consumption of sugars or ethanol and glucose in a Saccharomyces cerevisiae strain deleted in the HXK2 gene.

Autor: Raamsdonk LM; Swammerdam Institute for Life Science (SILS), Faculty of Science, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands., Diderich JA, Kuiper A, van Gaalen M, Kruckeberg AL, Berden JA, Van Dam K
Jazyk: angličtina
Zdroj: Yeast (Chichester, England) [Yeast] 2001 Aug; Vol. 18 (11), pp. 1023-33.
DOI: 10.1002/yea.746
Abstrakt: In previous studies it was shown that deletion of the HXK2 gene in Saccharomyces cerevisiae yields a strain that hardly produces ethanol and grows almost exclusively oxidatively in the presence of abundant glucose. This paper reports on physiological studies on the hxk2 deletion strain on mixtures of glucose/sucrose, glucose/galactose, glucose/maltose and glucose/ethanol in aerobic batch cultures. The hxk2 deletion strain co-consumed galactose and sucrose, together with glucose. In addition, co-consumption of glucose and ethanol was observed during the early exponential growth phase. In S.cerevisiae, co-consumption of ethanol and glucose (in the presence of abundant glucose) has never been reported before. The specific respiration rate of the hxk2 deletion strain growing on the glucose/ethanol mixture was 900 micromol.min(-1).(g protein)(-1), which is four to five times higher than that of the hxk2 deletion strain growing oxidatively on glucose, three times higher than its parent growing on ethanol (when respiration is fully derepressed) and is almost 10 times higher than its parent growing on glucose (when respiration is repressed). This indicates that the hxk2 deletion strain has a strongly enhanced oxidative capacity when grown on a mixture of glucose and ethanol.
(Copyright 2001 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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