Simplified microneutralization test for serotyping adenovirus isolates.

Autor: Malasig MD; Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, California 92186, USA. malasig@nhrc.navy.mil, Goswami PR, Crawford-Miksza LK, Schnurr DP, Gray GC
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2001 Aug; Vol. 39 (8), pp. 2984-6.
DOI: 10.1128/JCM.39.8.2984-2986.2001
Abstrakt: A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.
Databáze: MEDLINE