| Autor: |
Vandahl BB; Department of Medical Microbiology and Immunology, University of Aarhus, Denmark. vandahl@medmicro.au.dk, Gevaert K, Demol H, Hoorelbeke B, Holm A, Vandekerckhove J, Christiansen G, Birkelund S |
| Jazyk: |
angličtina |
| Zdroj: |
Electrophoresis [Electrophoresis] 2001 May; Vol. 22 (9), pp. 1697-704. |
| DOI: |
10.1002/1522-2683(200105)22:9<1697::AID-ELPS1697>3.0.CO;2-D |
| Abstrakt: |
Chlamydia pneumoniae is an obligate intracellular human pathogen infecting epithelial cells of the upper respiratory tract. It is a Gram-negative bacteria and has a unique biphasic developmental cycle. In this study, we use two-dimensional gel electrophoresis in combination with radioactive labeling to investigate time-dependent expression and processing of C. pneumoniae proteins. We report on (i) the identification of a hypothetical protein which is expressed late in the developmental cycle and subsequently processed; we speculate that this protein may be of importance for the developmental cycle of Chlamydia; (ii) the identification of the major outer membrane protein in three different variants, which may all be present in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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