| Autor: |
Bolken TC; SIGA Research Laboratories, Corvallis, Oregon 97333, USA., Franke CA, Zeller GO, Hruby DE |
| Jazyk: |
angličtina |
| Zdroj: |
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2001 Mar; Vol. 55 (2), pp. 192-7. |
| DOI: |
10.1007/s002530000519 |
| Abstrakt: |
A new intragenic chromosomal integration site within the lacG gene of the lac operon has been identified in Streptococcus gordonii for use in the expression of foreign genes. Introduction of a portion of the Streptococcus pyogenes emm6 gene into the lacG locus resulted in the lactose-inducible surface expression of the S. pyogenes M6 protein. This result demonstrates the ability to modulate the in vitro or in vivo expression of a foreign gene in a S. gordonii recombinant using a biosynthetic metabolite. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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