| Autor: |
Moran AJ; Department of Biochemistry and microbiology, University of Victoria, Victoria BC, Canada V8W 3P6, Brazil., Treit JD, Whitney JL, Abomoelak B, Houghton R, Skeiky YA, Sampaio DP, Badaró R, Nano FE |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 2001 Apr 20; Vol. 198 (1), pp. 31-6. |
| DOI: |
10.1111/j.1574-6968.2001.tb10615.x |
| Abstrakt: |
To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulmonary tuberculosis (EP-TB) or HIV-positive individuals with pulmonary tuberculosis (TBH). Specific and high level antibody responses were obtained for four recombinant proteins, of which antigen GST-822 was recognized by 60% of EP-TB and 42% of TBH and antigen MBP-506 was recognized by 45% of EP-TB and 61% of TBH. These results suggest that these proteins are strong candidates as subunits in a polyvalent serodiagnostic test. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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