| Autor: |
Hansen KM; Life Sciences Division, Oak Ridge National Laboratory, Tennessee 37831, USA., Ji HF, Wu G, Datar R, Cote R, Majumdar A, Thundat T |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2001 Apr 01; Vol. 73 (7), pp. 1567-71. |
| DOI: |
10.1021/ac0012748 |
| Abstrakt: |
Characterization of single-nucleotide polymorphisms is a major focus of current genomics research. We demonstrate the discrimination of DNA mismatches using an elegantly simple microcantilever-based optical deflection assay, without the need for external labeling. Gold-coated silicon AFM cantilevers were functionalized with thiolated 20- or 25-mer probe DNA oligonucleotides and exposed to target oligonucleotides of varying sequence in static and flow conditions. Hybridization of 10-mer complementary target oligonucleotides resulted in net positive deflection, while hybridization with targets containing one or two internal mismatches resulted in net negative deflection. Mismatched targets produced a stable and measurable signal when only a four-base pair stretch was complementary to the probe sequence. This technique is readily adaptable to a high-throughput array format and provides a distinct positive/negative signal for easy interpretation of oligonucleotide hybridization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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