| Autor: |
Moynihan JS; Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, NW1 0TU, London, UK., Jones DH, Farrar GH, Howard CR |
| Jazyk: |
angličtina |
| Zdroj: |
Vaccine [Vaccine] 2001 Apr 30; Vol. 19 (23-24), pp. 3292-300. |
| DOI: |
10.1016/s0264-410x(00)00540-5 |
| Abstrakt: |
A 48 amino acid synthetic peptide (S121/48) representing residues 121-167 of the major envelope protein of hepatitis B virus (HBsAg) was successfully encapsulated into polylactide co-glycolide microspheres. A single immunization of the microspheres in BALB/c (H-2d) mice resulted in the production of high-titre anti-HBs antibodies (IgG1-type). The response was long lasting and was superior to that obtained using the same peptide adjuvanted with Freund's complete adjuvant. A T-cell memory response was detected 10 weeks after a booster immunization (approximately 35 weeks after initial immunization) as measured by in-vitro re-stimulation of splenocytes. This study illustrates the feasibility of a single dose vaccine for hepatitis B and is, to our knowledge, the first demonstration of a synthetic peptide immunogen inducing anti-native protein antibodies of comparable titre to those obtained with conventional vaccines for hepatitis B. The suitability of a synthetic peptide vaccine for hepatitis B is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Full Text from ScienceDirect