| Autor: |
Fleeton MN; Microbiology and Tumorbiology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden., Chen M, Berglund P, Rhodes G, Parker SE, Murphy M, Atkins GJ, Liljeström P |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of infectious diseases [J Infect Dis] 2001 May 01; Vol. 183 (9), pp. 1395-8. Date of Electronic Publication: 2001 Mar 30. |
| DOI: |
10.1086/319857 |
| Abstrakt: |
In genetic vaccination, recipients are immunized with antigen-encoding nucleic acid, usually DNA. This study addressed the possibility of using the recombinant alpha virus RNA molecule, which replicates in the cytoplasm of transfected cells, as a novel approach for genetic vaccination. Mice were immunized with recombinant Semliki Forest virus RNA-encoding envelope proteins from one of 3 viruses: influenza A virus, a tickborne flavivirus (louping ill virus), or respiratory syncytial virus (RSV). Serologic analyses showed that antigen-specific antibody responses were elicited. IgG isotyping indicated that predominantly Th1 type immune responses were induced after immunization with RSV F protein-encoding RNA, which is relevant for protection against RSV infection. Challenge infection showed that RNA immunization had elicited significant levels of protection against the 3 model virus diseases. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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