| Autor: |
Choi MH; Bioanalysis and Biotransformation Research Center, KIST, P.O. Box 131, Cheongryang, Seoul 130-650, Korea., Chung BC |
| Jazyk: |
angličtina |
| Zdroj: |
The Analyst [Analyst] 2001 Mar; Vol. 126 (3), pp. 306-9. |
| DOI: |
10.1039/b007929o |
| Abstrakt: |
An improved screening method was developed for 3'-hydroxystanozolol and its 17-epimer in human urine involving gas chromatography-mass spectrometry (GC-MS) with N-isobutyloxycarbonyl (isoBOC) and O-trimethylsilyl (TMS) derivatization. A procedure was reported previously for the pentane extraction of many steroids from urine in doping control, but it was not suitable for the detection of stanozolol metabolites. Compared with the n-pentane extraction method, which gave a poor recovery (< 10%), isoBOC extraction resulted in a good recovery (> 80%). The sensitivity and specificity of mixed N-isoBOC-O-TMS derivatization were adequate for the detection of 3'-hydroxystanozolol and its 17-epimer when 3 ml of urine was used with spiking at a level of 2 ng ml-1. When applied to a stanozolol-positive urine sample, the proposed method allowed rapid and sensitive screening for the detection of 3'-hydroxystanozolol and its 17-epimer. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
