| Autor: |
Zatsepin TS; Chemical Department and Belozersky Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow V-234, 119899 Russia., Kachalova AV, Romanova EA, Stestenko DA, Gait MJ, Oretskaia TS |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2001 Jan-Feb; Vol. 27 (1), pp. 45-51. |
| DOI: |
10.1023/a:1009579002330 |
| Abstrakt: |
A new uridine derivative, 2'-O-(2,3-dihydroxypropyl)uridine, and its 3'-phosphoramidite were obtained for direct introduction into oligonucleotides during automated chemical synthesis. Oligonucleotides 10 to 15 nt long harboring 2'-O-(2,3-dihydroxypropyl)uridine residues were synthesized; periodate oxidation of these oligomers gave oligonucleotides containing 2'-O-(2-oxoethyl)uridine residues. The presence of a reactive aldehyde group in 2' position of the carbohydrate moiety was confirmed by the interaction with p-nitrophenylhydrazine and methionine methyl ester. The thermostability of DNA duplexes containing modified units is practically indistinguishable from that of the natural analogues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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