| Autor: |
Tumanova OIu, Kuvshinov VN, Azaev MSh, Masharskiĭ AE, Klimov NA, Kozlov AP, Il'ichev AA, Sandakhchiev LS |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2001 Jan-Feb; Vol. 35 (1), pp. 146-51. |
| Abstrakt: |
A phase peptide library was screened with virus-neutralizing monoclonal antibodies (MCA) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MCA 2F5 were selected by ELISA. Amino acid sequence analysis revealed a homology to region 662-671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in serum of immunized rabbits. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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