| Autor: |
Kirber MT; Department of Physiology and Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, MA 01655, USA., Etter EF, Bellve KA, Lifshitz LM, Tuft RA, Fay FS, Walsh JV, Fogarty KE |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of physiology [J Physiol] 2001 Mar 01; Vol. 531 (Pt 2), pp. 315-27. |
| DOI: |
10.1111/j.1469-7793.2001.0315i.x |
| Abstrakt: |
We recorded Ca2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions (2D and 3D). In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs. Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent distance from the cell surface was not significantly different from the sparks that cause STOCs. Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence was high and others where it was quite low. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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