| Autor: |
Wizemann TM; MedImmune, Inc., Gaithersburg, Maryland 20878, USA., Heinrichs JH, Adamou JE, Erwin AL, Kunsch C, Choi GH, Barash SC, Rosen CA, Masure HR, Tuomanen E, Gayle A, Brewah YA, Walsh W, Barren P, Lathigra R, Hanson M, Langermann S, Johnson S, Koenig S |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 2001 Mar; Vol. 69 (3), pp. 1593-8. |
| DOI: |
10.1128/IAI.69.3.1593-1598.2001 |
| Abstrakt: |
Microbial targets for protective humoral immunity are typically surface-localized proteins and contain common sequence motifs related to their secretion or surface binding. Exploiting the whole genome sequence of the human bacterial pathogen Streptococcus pneumoniae, we identified 130 open reading frames encoding proteins with secretion motifs or similarity to predicted virulence factors. Mice were immunized with 108 of these proteins, and 6 conferred protection against disseminated S. pneumoniae infection. Flow cytometry confirmed the surface localization of several of these targets. Each of the six protective antigens showed broad strain distribution and immunogenicity during human infection. Our results validate the use of a genomic approach for the identification of novel microbial targets that elicit a protective immune response. These new antigens may play a role in the development of improved vaccines against S. pneumoniae. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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