| Autor: |
Whitehurst CE; Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Schlissel MS, Chen J |
| Jazyk: |
angličtina |
| Zdroj: |
Immunity [Immunity] 2000 Nov; Vol. 13 (5), pp. 703-14. |
| DOI: |
10.1016/s1074-7613(00)00069-8 |
| Abstrakt: |
The role of the germline transcriptional promoter, PD beta 1, in V(D)J recombination at the T cell receptor beta locus was investigated. Deletion of PD beta 1 caused reduced germline transcription and DNA hypermethylation in the Dbeta1-J beta 1 region and decreased D beta 1 rearrangement. Analyses of methylation levels surrounding recombination signal sequences (RSS) before, during, and after recombination revealed that under physiological conditions cleavage of hypomethylated alleles was preferred over hypermethylated alleles. Methylation of a specific CpG site within the heptamer of the 3' D beta 1 RSS was incompatible with cleavage by the V(D)J recombinase. These findings suggest that methylation can regulate V(D)J recombination both at a general level by influencing regional chromatin accessibility and specifically by blocking RSS recognition or cleavage by the V(D)J recombinase. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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