| Autor: |
Beltran FF; Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, 30071, Murcia, Spain., Castillo R, Vicente-Soler J, Cansado J, Gacto M |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 2000 Dec 01; Vol. 193 (1), pp. 117-21. |
| DOI: |
10.1111/j.1574-6968.2000.tb09412.x |
| Abstrakt: |
Spores from Schizosaccharomyces pombe contain neutral and acid trehalases. When spores from strains disrupted for ntp1(+), which encodes neutral trehalase, were induced to germinate, the onset of the process was markedly delayed as compared to wild-type spores. Further outgrowth was also reduced. Dormant spores lacking neutral trehalase contained twice the amount of trehalose present in wild-type spores and mobilised the intracellular pool of trehalose at a slower rate during germination. Inhibition by phloridzin of the sporulation-specific acid trehalase in ntp1-disrupted spores arrested germination completely while prompting no effect on wild-type spores. These results suggest that the two trehalase enzymes may support the utilisation of trehalose during germination but neutral trehalase is required for a more rapid and efficient process. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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