| Autor: |
Bordo D; National Institute for Cancer Research c/o Advanced Biotechnology Center, Largo R. Benzi 10, 16132 Genova, Italy. domenico@alcor.ge.infm.it, Larson TJ, Donahue JL, Spallarossa A, Bolognesi M |
| Jazyk: |
angličtina |
| Zdroj: |
Acta crystallographica. Section D, Biological crystallography [Acta Crystallogr D Biol Crystallogr] 2000 Dec; Vol. 56 (Pt 12), pp. 1691-3. |
| DOI: |
10.1107/s0907444900015304 |
| Abstrakt: |
The Escherichia coli sn-glycerol 3-phosphate regulon contains the glpE gene coding for a 12 kDa protein which displays a sequence and a thiosulfate:cyanide sulfurtransferase activity similar to those of rhodanese enzymes. The GlpE protein was overexpressed, purified to homogeneity and crystallized in the trigonal space group P3(1) (or P3(2)). The unit-cell parameters are a = b = 53.87, c = 30.52 A, gamma = 120 degrees. Evaluation of the crystal packing parameter establishes the presence of one molecule per asymmetric unit, with a solvent content of 42%. The GlpE crystals display very high resolution diffraction; a 1.06 A data set was collected using synchrotron radiation (lambda = 0.9102 A) with an overall completeness of 99.6%. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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