| Autor: |
Shim H; Department of Chemical Engineering, University of Connecticut, Storrs, Connecticut 06269-3222, USA., Chauhan S, Ryoo D, Bowers K, Thomas SM, Canada KA, Burken JG, Wood TK |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2000 Nov; Vol. 66 (11), pp. 4673-8. |
| DOI: |
10.1128/AEM.66.11.4673-4678.2000 |
| Abstrakt: |
Indigenous bacteria from poplar tree (Populus canadensis var. eugenei 'Imperial Carolina') and southern California shrub rhizospheres, as well as two tree-colonizing Rhizobium strains (ATCC 10320 and ATCC 35645), were engineered to express constitutively and stably toluene o-monooxygenase (TOM) from Burkholderia cepacia G4 by integrating the tom locus into the chromosome. The poplar and Rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and were competitive against the unengineered hosts in wheat and barley rhizospheres for 1 month (colonization occurred at a level of 1.0 x 10(5) to 23 x 10(5) CFU/cm of root). In addition, six of these recombinants colonized poplar roots stably and competitively with populations as large as 79% +/- 12% of all rhizosphere bacteria after 28 days (0.2 x 10(5) to 31 x 10(5) CFU/cm of root). Furthermore, five of the most competitive poplar recombinants (e.g., Pb3-1 and Pb5-1, which were identified as Pseudomonas sp. strain PsK recombinants) retained the ability to express TOM for 29 days as 100% +/- 0% of the recombinants detected in the poplar rhizosphere expressed TOM constitutively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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