| Autor: |
Baumgardner JE; Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania 19104-4283, USA. jbaumgar@mail.med.upenn.edu, Choi IC, Vonk-Noordegraaf A, Frasch HF, Neufeld GR, Marshall BE |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2000 Nov; Vol. 89 (5), pp. 1699-708. |
| DOI: |
10.1152/jappl.2000.89.5.1699 |
| Abstrakt: |
We developed micropore membrane inlet mass spectrometer (MMIMS) probes to rapidly measure inert-gas partial pressures in small blood samples. The mass spectrometer output was linearly related to inert-gas partial pressure (r(2) of 0.996-1.000) and was nearly independent of large variations in inert-gas solubility in liquid samples. We infused six inert gases into five pentobarbital-anesthetized New Zealand rabbits and used the MMIMS system to measure inert-gas partial pressures in systemic and pulmonary arterial blood and in mixed expired gas samples. The retention and excretion data were transformed into distributions of ventilation-to-perfusion ratios (V(A)/Q) with the use of linear regression techniques. Distributions of V(A)/Q were unimodal and broad, consistent with prior reports in the normal rabbit. Total blood sample volume for each VA/Q distribution was 4 ml, and analysis time was 8 min. MMIMS provides a convenient method to perform the multiple inert-gas elimination technique rapidly and with small blood sample volumes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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