| Autor: |
Khusseĭn IA; Timiryazev Agricultural Academy, Russian Academy of Sciences, Moscow, Russia., Kochneva EZ, Khadeeva NV |
| Jazyk: |
ruština |
| Zdroj: |
Genetika [Genetika] 2000 Aug; Vol. 36 (8), pp. 1093-9. |
| Abstrakt: |
Nodular segments of the stem and stolons of aseptic Stachys sieboldii (Miq.) plants were cultured in Gamborg's B5 medium at a low (1-2 mg/l) or high (10 mg/l) concentration of cytokinins (6-BAP, kinetin) and produced many adventitious buds and shoots without callus formation. Several plants were regenerated from nodular segments incubated at various concentrations of 5-bromodeoxiuridine (5-BdU) for three weeks. All regenerants were morphologically normal. Peroxidase electrophoretic patterns were analyzed to determine whether somaclonal variation is associated with S. sieboldii micropropagation. The frequency of changed forms was higher with the high 6-BAP concentration and with mutagenic treatment. Changed forms were not detected in plants grown at a low cytokinin concentration, which can be used in S. sieboldii micropropagation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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