| Autor: |
Yusa T; Department of Anesthesiology, University of the Ryukyus, Faculty of Medicine 207 Uehara, Nishihara-cho, Nakagami-gun, 903-0215, Okinawa, Japan. t1935y@med.u-ryuku.ac.jp |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroscience letters [Neurosci Lett] 2000 Oct 27; Vol. 293 (2), pp. 123-6. |
| DOI: |
10.1016/s0304-3940(00)01501-9 |
| Abstrakt: |
Apart from its physiological role as a major antioxidant, ascorbate is highly concentrated in neuropils and ascorbate-mediated protection from excitotoxins has been demonstrated in vitro. Therefore, extracellular release of ascorbate during the early stage of ischemia-reperfusion was measured using a microdialysis electrode technique. One or two probes of the microdialysis biosensor were inserted into the rat striatum. One probe (n=16) was perfused with phosphate-buffered saline (PBS) for continuous oxidative signal recording. A second electropolymerised probe inserted into the other side of the striatum was perfused with PBS containing ascorbate oxidase in six rats. Forebrain ischemia-reperfusion was performed for 10min, followed by reperfusion for 60min. Ascorbate increased transiently during ischemia, and markedly to a maximum of 247.5+/-55. 8 microM from the baseline of 68.5+/-25.3 microM after reperfusion. The marked increase of extracellular ascorbate may be a marker of the early stage of reperfusion. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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